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Complete kits designed to lyse cells and detect beta-galactosidase reporter protein activity in the prepared cell lysates, which can be obtained from mammals, yeasts, and other species. Also includes colorimetric and fluorometric detection assay reagents.
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β-Galactosidase (EC 3.2.1.23) is a glycoside hydrolase that breaks down β-glycosidic bonds between galactose and its organic parts. In E. coli, the lacZ gene is the structural gene for β-galactosidase, forming part of the lac operon's induction system. This enzyme can hydrolyze lactose into glucose and galactose, which then enter glycolysis.
Can catalyze the transgalactosylation of lactose into allolactose
Allolactose can further be broken into monosaccharides
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MW 301.25 g/mol. β-galactosidase substrate for colorimetric and EIA applications. Hydrolysed by β-galactosidase to yield galactose and ortho-Nitrophenol, which is yellow in color.
Galactosidase Alpha Primary Antibody Unconjugated Public Immunogen Range 10-50/184 Host Species Rabbit Target Species Human Clonality Monoclonal Applications WB FCM Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Differentiate slow lactose fermenters and non-fermenters. Demonstration of beta-galactosidase accomplished by hydrolization of substrate that liberates ortho-nitrophenol for a characteristic yellow color. Pack of 28 tablets per tube.
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